What do you get when you cross a day job as a Medical Histopathologist with an interest in 3D printing and programming? You get a fully-baked Open Source microscope, specifically the Portable Upgradeable Modular Affordable (or PUMA), that’s what. And this is no toy microscope. By combining a sprinkle of off-the-shelf electronics available from pretty much anywhere, a pound or two of filament, and a dash of high quality optical parts, PUMA cooks up quite possibly one of the best open source microscopy experiences we’ve ever tasted.

GitHub user [TadPath] works as a medical pathologist and clearly knows a thing or two about what makes a great instrument, so it is a genuine joy for us to see this tasty project laid out in such a complete fashion. Many a time we’ve looked into an high-profile project, only to find a pile of STL files and some hard to source special parts. But not here. This is deliberately designed to be buildable by practically anyone with access to a 3D printer and an eBay account.

The project is not currently certified for medical diagnostics use, but that is likely only a matter of money and time. The value for education and research (especially in developing nations) cannot really be overstated.

The modularity allows a wide range of configurations from simple ambient light illumination, with a single objective, great for using out in the field without electricity, right up to a trinocular setup with TFT-based spatial light modulator enabling advanced methods such as Schlieren phase contrast (which allows visualisation of fluid flow inside a live cell, for example) and a heads-up display for making measurements from the sample. Add into the mix that PUMA is specifically designed to be quickly and easily broken down in the field, that helps busy researchers on the go, out in the sticks.

The GitHub repo has all the details you could need to build your own configuration and appropriate add-ons, everything from CAD files (FreeCAD source, so you can remix it to your heart’s content) and a detailed Bill-of-Materials for sourcing parts.

We covered fluorescence microscopy before, as well as many many other microscope related stories over the years, because quite simply, microscopes are a very important topic. Heck, this humble scribe has a binocular and a trinocular microscope on the bench next to him, and doesn’t even consider that unusual. If you’re hungry for an easily hackable, extendable and cost-effective scope, then this may be just the dish you were looking for.

Thanks to [linus] for the delicious tip!

We’ve seen a lot of interest in LSM (LASER Scanning Microscopes) lately. [Stoppi71] uses an Arduino, a CD drive, and–of all things–two speakers in his build. The speakers are used to move the sample by very small amounts.

The speakers create motion in the X and Y axis depending on the voltage fed to them via a digital analog converter. [Stoppi71] claims this technique can produce motion in the micron range. His results seem to prove that out. You can see a video about the device, below.

Oddly enough, [Stoppi71] found that older CD drives were easier to work with because they were not as miniaturized as more modern versions. The device uses the Arduino to drive the scanning table (the speakers), and read the photodetector. The results of the scan appear on an LCD screen.

Using a calibration slide from eBay, [Stoppi71] calibrated the device for different magnifications. You can see the test slide at medium magnification. For the record, a human hair is about 40 or 50 microns thick. So the 10 micron mark in the photo would be like splitting a hair in quarters or fifths.

The real goal was to view pits on CDs, and the instrument is more than capable of doing that. The image doesn’t show up all at once (it is scanning, after all) and it isn’t the kind of view you’d expect from an optical microscope, but a typical optical scope can’t resolve below about 200 microns. Special techniques can push that lower, but being able to resolve things at the one or two micron level with something this simple is a great accomplishment.

We recently saw a different-looking LSM built on a conventional microscope stage and a DVD drive. If LSM isn’t enough for you, maybe you should pitch in on the open source electron microscope project.

Remember that feeling when you first looked down on a microscope? Now you can re-live it but in slightly different way. [Venkes] came up with a way to make a Laser Scanning Microscope (LSM) with mostly off the shelf components that you probably have sitting around, collecting dust in your garage. He did it using some modified DVD pick-ups, an Arduino Uno, a laser and a LDR.

To be honest, there’s some more stuff involved in the making of the LSM but [Venkes] did a detailed Instructable explaining how everything fits together. You will need a fair dose of patience, it’s not very easy to get the focus right and it’s quite slow, an image takes about half an hour to complete, but it can do 1300x amplification at 65k pixels (256×256). From reading the instructions it seems that you will need a steady hand to assemble it together, some steps look kind of tricky. On the software side, the LSM uses Arduino and Processing. The Arduino part is responsible for the steering of the lens and taking the LDR readings. This information is then sent to Processing which takes care of interpreting the data and translate it to an image.

The build difficulty level should be between the DIY Smartphone Microscope and the Laser Sequencer Super Microscope. In the end, if everything goes right, you will end up with some cool images: